Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10636 | 3' | -57.8 | NC_002702.1 | + | 13350 | 0.66 | 0.433151 |
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Target: 5'- aUCAGggGCAggaGCUCCGCCCC-CACc -3' miRNA: 3'- -GGUCa-UGUggaCGAGGCGGGGuGUGc -5' |
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| 10636 | 3' | -57.8 | NC_002702.1 | + | 15205 | 0.67 | 0.395104 |
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Target: 5'- ---cUGCGCCUGCgaguagUCCGCCUCACccuggACGa -3' miRNA: 3'- ggucAUGUGGACG------AGGCGGGGUG-----UGC- -5' |
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| 10636 | 3' | -57.8 | NC_002702.1 | + | 12784 | 0.67 | 0.395104 |
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Target: 5'- gUCAG-ACACCUcgacGCcguucugCCGCCCCACGg- -3' miRNA: 3'- -GGUCaUGUGGA----CGa------GGCGGGGUGUgc -5' |
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| 10636 | 3' | -57.8 | NC_002702.1 | + | 32515 | 0.7 | 0.252431 |
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Target: 5'- -gGGUcACACg-GCUCCGCCCCuuACGu -3' miRNA: 3'- ggUCA-UGUGgaCGAGGCGGGGugUGC- -5' |
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| 10636 | 3' | -57.8 | NC_002702.1 | + | 107 | 0.7 | 0.252431 |
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Target: 5'- -gGGUcACACg-GCUCCGCCCCuuACGu -3' miRNA: 3'- ggUCA-UGUGgaCGAGGCGGGGugUGC- -5' |
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| 10636 | 3' | -57.8 | NC_002702.1 | + | 13256 | 1.1 | 0.000277 |
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Target: 5'- uCCAGUACACCUGCUCCGCCCCACACGu -3' miRNA: 3'- -GGUCAUGUGGACGAGGCGGGGUGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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