Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10637 | 3' | -58.3 | NC_002702.1 | + | 7493 | 0.66 | 0.385907 |
Target: 5'- ---aGGCGCUgagGGUGgCUCUCUUGGCc -3' miRNA: 3'- aaugCCGCGA---CCGUgGGGAGAAUCGc -5' |
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10637 | 3' | -58.3 | NC_002702.1 | + | 18836 | 0.66 | 0.350013 |
Target: 5'- -aGCGGCGCUGGCaaACCCUg---AGa- -3' miRNA: 3'- aaUGCCGCGACCG--UGGGGagaaUCgc -5' |
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10637 | 3' | -58.3 | NC_002702.1 | + | 14051 | 0.67 | 0.323871 |
Target: 5'- --cCGGCGgUGGUcgucuccGCCCCUCgucgcAGCGc -3' miRNA: 3'- aauGCCGCgACCG-------UGGGGAGaa---UCGC- -5' |
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10637 | 3' | -58.3 | NC_002702.1 | + | 10528 | 0.67 | 0.316559 |
Target: 5'- -aACGGcCGCUggguuuggGGCGCCCCggCgacGGCGg -3' miRNA: 3'- aaUGCC-GCGA--------CCGUGGGGa-Gaa-UCGC- -5' |
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10637 | 3' | -58.3 | NC_002702.1 | + | 19496 | 0.67 | 0.308581 |
Target: 5'- -aACGGuCGCcauugagGGCGCUCCUCUUaAGUa -3' miRNA: 3'- aaUGCC-GCGa------CCGUGGGGAGAA-UCGc -5' |
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10637 | 3' | -58.3 | NC_002702.1 | + | 14752 | 1.07 | 0.00028 |
Target: 5'- gUUACGGCGCUGGCACCCCUCUUAGCGg -3' miRNA: 3'- -AAUGCCGCGACCGUGGGGAGAAUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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