miRNA: 3'- uGGUGGGCGGuUGCCG--UGCGGG-------CCGU- -5'
miRNA: 3'- ugGUGGGCGGU-------------UGCCgU--------------GCGGGCCGU- -5'
Results 21 - 22 of 22 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1064 | 3' | -63.9 | NC_000929.1 | + | 30102 | 0.66 | 0.206623 |
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Target: 5'- gUCGCCUGUUuGCGGCgcACGCCCcagaaccGGCAg -3' miRNA: 3'- uGGUGGGCGGuUGCCG--UGCGGG-------CCGU- -5' |
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| 1064 | 3' | -63.9 | NC_000929.1 | + | 30833 | 0.69 | 0.133883 |
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Target: 5'- -aCACCUGCCAccgcacggauaauACGGgAgggggcaagugcgcCGCCCGGCAu -3' miRNA: 3'- ugGUGGGCGGU-------------UGCCgU--------------GCGGGCCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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