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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10641 | 5' | -47.7 | NC_002702.1 | + | 16203 | 0.66 | 0.944329 |
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Target: 5'- --cCCGCGUCCGUgAcuUCCgcacccACAGc -3' miRNA: 3'- gaaGGUGCAGGCAgUuuAGGau----UGUC- -5' |
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| 10641 | 5' | -47.7 | NC_002702.1 | + | 7149 | 0.67 | 0.927106 |
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Target: 5'- --aCCACGUCCaccaCAAAUCCUcuGACuGg -3' miRNA: 3'- gaaGGUGCAGGca--GUUUAGGA--UUGuC- -5' |
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| 10641 | 5' | -47.7 | NC_002702.1 | + | 1303 | 0.67 | 0.906926 |
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Target: 5'- --aCCugGUCUGUCAGAagCaUAAUAGa -3' miRNA: 3'- gaaGGugCAGGCAGUUUagG-AUUGUC- -5' |
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| 10641 | 5' | -47.7 | NC_002702.1 | + | 16003 | 0.68 | 0.857992 |
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Target: 5'- -cUCCugGUUCGcCAGAUCC--ACGGc -3' miRNA: 3'- gaAGGugCAGGCaGUUUAGGauUGUC- -5' |
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| 10641 | 5' | -47.7 | NC_002702.1 | + | 22357 | 0.69 | 0.848801 |
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Target: 5'- -gUCCu--UUCGUCAAAUCCUGAcCAGa -3' miRNA: 3'- gaAGGugcAGGCAGUUUAGGAUU-GUC- -5' |
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| 10641 | 5' | -47.7 | NC_002702.1 | + | 20934 | 0.94 | 0.03648 |
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Target: 5'- cCUUCCACGUCCGUCAAAUCCUcaaagcuccuucugGAUAGa -3' miRNA: 3'- -GAAGGUGCAGGCAGUUUAGGA--------------UUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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