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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10642 | 3' | -56.8 | NC_002702.1 | + | 10501 | 0.67 | 0.428661 |
Target: 5'- -------gCGACGGCGGCGCUGAgGAa -3' miRNA: 3'- cuacagagGCUGUCGUCGCGGCUgCU- -5' |
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10642 | 3' | -56.8 | NC_002702.1 | + | 10610 | 0.69 | 0.304932 |
Target: 5'- --gGUUUCUGGCGGuCAGCGCC-AUGAu -3' miRNA: 3'- cuaCAGAGGCUGUC-GUCGCGGcUGCU- -5' |
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10642 | 3' | -56.8 | NC_002702.1 | + | 14017 | 0.7 | 0.277891 |
Target: 5'- cGUGaCUCaGACAGCAcccgcggcccccgccGCGCCGGCGGu -3' miRNA: 3'- cUACaGAGgCUGUCGU---------------CGCGGCUGCU- -5' |
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10642 | 3' | -56.8 | NC_002702.1 | + | 16140 | 0.76 | 0.103686 |
Target: 5'- ---cUCUCC-ACAGUAGCGCCGACGc -3' miRNA: 3'- cuacAGAGGcUGUCGUCGCGGCUGCu -5' |
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10642 | 3' | -56.8 | NC_002702.1 | + | 21115 | 1.08 | 0.000416 |
Target: 5'- gGAUGUCUCCGACAGCAGCGCCGACGAc -3' miRNA: 3'- -CUACAGAGGCUGUCGUCGCGGCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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