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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10644 | 5' | -60.5 | NC_002702.1 | + | 8209 | 0.7 | 0.164014 |
Target: 5'- aGCGaCGGCUGcCCUGACCUcuucuucgaAGCUGGu -3' miRNA: 3'- gCGCgGUCGGC-GGACUGGA---------UCGACUc -5' |
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10644 | 5' | -60.5 | NC_002702.1 | + | 12874 | 0.66 | 0.289977 |
Target: 5'- uGCGCCaucgauuucaccaccAGCCGCCUcaacaACCU-GUUGGGg -3' miRNA: 3'- gCGCGG---------------UCGGCGGAc----UGGAuCGACUC- -5' |
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10644 | 5' | -60.5 | NC_002702.1 | + | 23875 | 1.09 | 0.000125 |
Target: 5'- aCGCGCCAGCCGCCUGACCUAGCUGAGg -3' miRNA: 3'- -GCGCGGUCGGCGGACUGGAUCGACUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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