miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10646 3' -52.1 NC_002702.1 + 14561 0.66 0.779441
Target:  5'- -aUCUCGuCGGCCUGucucuuAAAGCCg -3'
miRNA:   3'- ggAGAGC-GCCGGAUucaaccUUUCGG- -5'
10646 3' -52.1 NC_002702.1 + 14736 0.66 0.747136
Target:  5'- cCCUCUUaGCGGCCUggcGAGgcgugacgacGGgcAGCUg -3'
miRNA:   3'- -GGAGAG-CGCCGGA---UUCaa--------CCuuUCGG- -5'
10646 3' -52.1 NC_002702.1 + 5130 0.66 0.736089
Target:  5'- gCUUCUUcgGCGGCCUcauuguccucGUUGGAGAcGUCg -3'
miRNA:   3'- -GGAGAG--CGCCGGAuu--------CAACCUUU-CGG- -5'
10646 3' -52.1 NC_002702.1 + 22202 0.67 0.702304
Target:  5'- -aUCUCGCcuGGCCUcccccuuuaagGAGUugUGGAGuacguguuGGCCg -3'
miRNA:   3'- ggAGAGCG--CCGGA-----------UUCA--ACCUU--------UCGG- -5'
10646 3' -52.1 NC_002702.1 + 18302 0.69 0.575131
Target:  5'- cCUUCUCGCaacugGGCCgccuuuagGGGUUGGAGuuuuacgcGCCu -3'
miRNA:   3'- -GGAGAGCG-----CCGGa-------UUCAACCUUu-------CGG- -5'
10646 3' -52.1 NC_002702.1 + 7854 0.7 0.529687
Target:  5'- aCCUgaaCGCGGCUcaUGAuGUUGGAgaAGGCCu -3'
miRNA:   3'- -GGAga-GCGCCGG--AUU-CAACCU--UUCGG- -5'
10646 3' -52.1 NC_002702.1 + 8402 1.14 0.000544
Target:  5'- cCCUCUCGCGGCCUAAGUUGGAAAGCCc -3'
miRNA:   3'- -GGAGAGCGCCGGAUUCAACCUUUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.