Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10646 | 3' | -52.1 | NC_002702.1 | + | 14561 | 0.66 | 0.779441 |
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Target: 5'- -aUCUCGuCGGCCUGucucuuAAAGCCg -3' miRNA: 3'- ggAGAGC-GCCGGAUucaaccUUUCGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 14736 | 0.66 | 0.747136 |
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Target: 5'- cCCUCUUaGCGGCCUggcGAGgcgugacgacGGgcAGCUg -3' miRNA: 3'- -GGAGAG-CGCCGGA---UUCaa--------CCuuUCGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 5130 | 0.66 | 0.736089 |
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Target: 5'- gCUUCUUcgGCGGCCUcauuguccucGUUGGAGAcGUCg -3' miRNA: 3'- -GGAGAG--CGCCGGAuu--------CAACCUUU-CGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 22202 | 0.67 | 0.702304 |
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Target: 5'- -aUCUCGCcuGGCCUcccccuuuaagGAGUugUGGAGuacguguuGGCCg -3' miRNA: 3'- ggAGAGCG--CCGGA-----------UUCA--ACCUU--------UCGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 18302 | 0.69 | 0.575131 |
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Target: 5'- cCUUCUCGCaacugGGCCgccuuuagGGGUUGGAGuuuuacgcGCCu -3' miRNA: 3'- -GGAGAGCG-----CCGGa-------UUCAACCUUu-------CGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 7854 | 0.7 | 0.529687 |
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Target: 5'- aCCUgaaCGCGGCUcaUGAuGUUGGAgaAGGCCu -3' miRNA: 3'- -GGAga-GCGCCGG--AUU-CAACCU--UUCGG- -5' |
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| 10646 | 3' | -52.1 | NC_002702.1 | + | 8402 | 1.14 | 0.000544 |
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Target: 5'- cCCUCUCGCGGCCUAAGUUGGAAAGCCc -3' miRNA: 3'- -GGAGAGCGCCGGAUUCAACCUUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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