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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10646 | 5' | -55.3 | NC_002702.1 | + | 8437 | 1.06 | 0.000685 |
Target: 5'- uAAGGCCUCUCCAAAGCAAGACGGGAGu -3' miRNA: 3'- -UUCCGGAGAGGUUUCGUUCUGCCCUC- -5' |
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10646 | 5' | -55.3 | NC_002702.1 | + | 8032 | 0.66 | 0.525757 |
Target: 5'- -uGGCCUCUCCAuu------CGGGAGa -3' miRNA: 3'- uuCCGGAGAGGUuucguucuGCCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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