Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10649 | 3' | -57 | NC_002702.1 | + | 10526 | 0.66 | 0.472481 |
Target: 5'- -cGGCcgcugGGUUugGGGCG-CCCCGgcgacGGCg -3' miRNA: 3'- uaCUG-----CUAGugCCUGCuGGGGCa----CCG- -5' |
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10649 | 3' | -57 | NC_002702.1 | + | 14111 | 0.68 | 0.375865 |
Target: 5'- -cGACGAgggGCGGAgaCGACCaCCGccGGCg -3' miRNA: 3'- uaCUGCUag-UGCCU--GCUGG-GGCa-CCG- -5' |
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10649 | 3' | -57 | NC_002702.1 | + | 15602 | 0.7 | 0.278819 |
Target: 5'- --aGCGAUgCcCGGaACGGCCCCGaUGGCc -3' miRNA: 3'- uacUGCUA-GuGCC-UGCUGGGGC-ACCG- -5' |
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10649 | 3' | -57 | NC_002702.1 | + | 101 | 0.82 | 0.041438 |
Target: 5'- -cGGCGG--GCGGGCGcGCCCCGUGGCg -3' miRNA: 3'- uaCUGCUagUGCCUGC-UGGGGCACCG- -5' |
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10649 | 3' | -57 | NC_002702.1 | + | 32521 | 0.82 | 0.041438 |
Target: 5'- -cGGCGG--GCGGGCGcGCCCCGUGGCg -3' miRNA: 3'- uaCUGCUagUGCCUGC-UGGGGCACCG- -5' |
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10649 | 3' | -57 | NC_002702.1 | + | 13784 | 1.08 | 0.00041 |
Target: 5'- cAUGACGAUCACGGACGACCCCGUGGCc -3' miRNA: 3'- -UACUGCUAGUGCCUGCUGGGGCACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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