miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10649 5' -64.7 NC_002702.1 + 13821 1.08 0.000081
Target:  5'- aACGCCAUCGGCCCUGGCCGGAGGCGGc -3'
miRNA:   3'- -UGCGGUAGCCGGGACCGGCCUCCGCC- -5'
10649 5' -64.7 NC_002702.1 + 12694 0.73 0.059716
Target:  5'- aAUGCCAUcaCGGagcacuaUCUGGCCGuGGGGCGGc -3'
miRNA:   3'- -UGCGGUA--GCCg------GGACCGGC-CUCCGCC- -5'
10649 5' -64.7 NC_002702.1 + 14251 0.66 0.193527
Target:  5'- gGCGCCAgagaucuaUGGCCg-GcGCCGGAGG-GGu -3'
miRNA:   3'- -UGCGGUa-------GCCGGgaC-CGGCCUCCgCC- -5'
10649 5' -64.7 NC_002702.1 + 15995 0.69 0.113268
Target:  5'- uCGCCAgauccaCGGCgCCgUGGauaCCGGAGGCGa -3'
miRNA:   3'- uGCGGUa-----GCCG-GG-ACC---GGCCUCCGCc -5'
10649 5' -64.7 NC_002702.1 + 14922 0.71 0.08107
Target:  5'- uGCGCCGUcugggccuagcaaCGGCCCUGGCgGGcaaaagaaAGGCc- -3'
miRNA:   3'- -UGCGGUA-------------GCCGGGACCGgCC--------UCCGcc -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.