miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
10652 5' -49.4 NC_002702.1 + 9853 0.66 0.858029
Target:  5'- -uGGGACUUugUggAGGCguaugUGGGUAACc -3'
miRNA:   3'- guCUCUGGAugAaaUUCG-----ACCCGUUG- -5'
10652 5' -49.4 NC_002702.1 + 22125 0.66 0.848748
Target:  5'- aGGAGACg------AGGUUGGGCAGCu -3'
miRNA:   3'- gUCUCUGgaugaaaUUCGACCCGUUG- -5'
10652 5' -49.4 NC_002702.1 + 13915 0.7 0.623439
Target:  5'- cCAGAGcCCUGCUgcggcGAGC-GaGGCGACg -3'
miRNA:   3'- -GUCUCuGGAUGAaa---UUCGaC-CCGUUG- -5'
10652 5' -49.4 NC_002702.1 + 24893 0.7 0.621014
Target:  5'- aAGuGACCgccuugaugcgcACUUcuGGCUGGGCGGCu -3'
miRNA:   3'- gUCuCUGGa-----------UGAAauUCGACCCGUUG- -5'
10652 5' -49.4 NC_002702.1 + 16534 1.12 0.001094
Target:  5'- aCAGAGACCUACUUUAAGCUGGGCAACa -3'
miRNA:   3'- -GUCUCUGGAUGAAAUUCGACCCGUUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.