Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10655 | 3' | -56.9 | NC_002702.1 | + | 22748 | 1.07 | 0.000452 |
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Target: 5'- cACACCCACUCAAACUCACGGCCGGCGc -3' miRNA: 3'- -UGUGGGUGAGUUUGAGUGCCGGCCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 13689 | 0.73 | 0.161357 |
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Target: 5'- gGCGCCCGCUCcauguccgAGACUCACccgguGCUGGUGa -3' miRNA: 3'- -UGUGGGUGAG--------UUUGAGUGc----CGGCCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 14741 | 0.69 | 0.332111 |
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Target: 5'- gGCACCC-CUCuua---GCGGCCuGGCGa -3' miRNA: 3'- -UGUGGGuGAGuuugagUGCCGG-CCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 10881 | 0.68 | 0.375442 |
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Target: 5'- aACGCCUucCUCAGcACUCu--GCCGGCGa -3' miRNA: 3'- -UGUGGGu-GAGUU-UGAGugcCGGCCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 10436 | 0.68 | 0.375442 |
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Target: 5'- cCGCCCACUC-----UAUGGCCGGaCGa -3' miRNA: 3'- uGUGGGUGAGuuugaGUGCCGGCC-GC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 16581 | 0.68 | 0.384555 |
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Target: 5'- uGCGCCCACUCAcgauguGAC-CACGGagcguucgcagCGGCu -3' miRNA: 3'- -UGUGGGUGAGU------UUGaGUGCCg----------GCCGc -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 14029 | 0.67 | 0.403214 |
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Target: 5'- aGCACCCGCggcccccGC-CGC-GCCGGCGg -3' miRNA: 3'- -UGUGGGUGaguu---UGaGUGcCGGCCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 9007 | 0.67 | 0.412756 |
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Target: 5'- uGCACCUGCUCcguguACUUgagGCGGCUguaGGCGc -3' miRNA: 3'- -UGUGGGUGAGuu---UGAG---UGCCGG---CCGC- -5' |
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| 10655 | 3' | -56.9 | NC_002702.1 | + | 13974 | 0.66 | 0.483227 |
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Target: 5'- uCGCCuCGCUCGccgcagcagGGCUC-UGGCCGcGCGc -3' miRNA: 3'- uGUGG-GUGAGU---------UUGAGuGCCGGC-CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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