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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10656 | 5' | -54.5 | NC_002702.1 | + | 15102 | 0.66 | 0.547805 |
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Target: 5'- cAUCCgUCUUcACCCCUcCCAGCUAg-- -3' miRNA: 3'- uUAGGgAGGA-UGGGGA-GGUUGGUaag -5' |
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| 10656 | 5' | -54.5 | NC_002702.1 | + | 7639 | 0.66 | 0.582314 |
|
Target: 5'- uGUCCCU-CUGCCCCgagaagaCGACCGgacccgUCa -3' miRNA: 3'- uUAGGGAgGAUGGGGag-----GUUGGUa-----AG- -5' |
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| 10656 | 5' | -54.5 | NC_002702.1 | + | 10348 | 0.68 | 0.438749 |
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Target: 5'- --gCCCgccugcgcagCCUACCCCguUCCGGCCucgUCg -3' miRNA: 3'- uuaGGGa---------GGAUGGGG--AGGUUGGua-AG- -5' |
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| 10656 | 5' | -54.5 | NC_002702.1 | + | 28823 | 0.72 | 0.256473 |
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Target: 5'- --aCCCUCUgcuuCCUCUCCAACCAc-- -3' miRNA: 3'- uuaGGGAGGau--GGGGAGGUUGGUaag -5' |
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| 10656 | 5' | -54.5 | NC_002702.1 | + | 9629 | 0.72 | 0.249549 |
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Target: 5'- --cCCCUCCUcCCCCUCCcGCUcccuaaaagaGUUCg -3' miRNA: 3'- uuaGGGAGGAuGGGGAGGuUGG----------UAAG- -5' |
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| 10656 | 5' | -54.5 | NC_002702.1 | + | 23237 | 1.06 | 0.000754 |
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Target: 5'- aAAUCCCUCCUACCCCUCCAACCAUUCc -3' miRNA: 3'- -UUAGGGAGGAUGGGGAGGUUGGUAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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