Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1066 | 5' | -50.6 | NC_000929.1 | + | 27390 | 1.12 | 0.001277 |
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Target: 5'- cGGACACACUUUCAAAUACCGGGGGCGu -3' miRNA: 3'- -CCUGUGUGAAAGUUUAUGGCCCCCGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 18672 | 0.77 | 0.282704 |
|
Target: 5'- uGGACACGCUggaaauuAAUGCCGGGaaaguGGCGg -3' miRNA: 3'- -CCUGUGUGAaagu---UUAUGGCCC-----CCGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 31016 | 0.68 | 0.747701 |
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Target: 5'- uGAUGCGCgg-CAAAUGCUGcGGGaGCGu -3' miRNA: 3'- cCUGUGUGaaaGUUUAUGGC-CCC-CGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 14961 | 0.68 | 0.779986 |
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Target: 5'- gGGGCugGCUgggaaaaugCGcGUGCCGGuGGCGc -3' miRNA: 3'- -CCUGugUGAaa-------GUuUAUGGCCcCCGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 7607 | 0.68 | 0.73666 |
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Target: 5'- aGGGCACuCUUUCAGAUAUucaGGGGa-- -3' miRNA: 3'- -CCUGUGuGAAAGUUUAUGg--CCCCcgc -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 29510 | 0.67 | 0.820582 |
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Target: 5'- uGACACGCUgccggauggUUCAGAUgaucGCCGuggaugguGGGGUGa -3' miRNA: 3'- cCUGUGUGA---------AAGUUUA----UGGC--------CCCCGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 610 | 0.67 | 0.820582 |
|
Target: 5'- aGGCACugaaACUUUUAAGUAaaGGGGGg- -3' miRNA: 3'- cCUGUG----UGAAAGUUUAUggCCCCCgc -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 23914 | 0.66 | 0.848755 |
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Target: 5'- cGGCAuCGCUgacaCAAGaa-CGGGGGCGa -3' miRNA: 3'- cCUGU-GUGAaa--GUUUaugGCCCCCGC- -5' |
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| 1066 | 5' | -50.6 | NC_000929.1 | + | 27365 | 0.66 | 0.848755 |
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Target: 5'- -aAC-CACUgcCA--UGCUGGGGGCGg -3' miRNA: 3'- ccUGuGUGAaaGUuuAUGGCCCCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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