Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10660 | 5' | -69.6 | NC_002702.1 | + | 10332 | 0.66 | 0.096504 |
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Target: 5'- gACGaggaGGGGCaGgGCCCGCCUGCg- -3' miRNA: 3'- -UGCggugCCCCG-CgCGGGCGGGCGgc -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 10456 | 0.67 | 0.091212 |
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Target: 5'- aGCGCCGCcgucgccGGGGCGC-CCCaaaCCCagcgGCCGu -3' miRNA: 3'- -UGCGGUG-------CCCCGCGcGGGc--GGG----CGGC- -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 13842 | 0.67 | 0.089029 |
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Target: 5'- -gGCCACGGGGU-CGUCCGUgaUCGUCa -3' miRNA: 3'- ugCGGUGCCCCGcGCGGGCG--GGCGGc -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 11140 | 0.68 | 0.0757 |
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Target: 5'- cCGCCACGGGG-GCGUUCaCCCcCCa -3' miRNA: 3'- uGCGGUGCCCCgCGCGGGcGGGcGGc -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 14431 | 0.68 | 0.067716 |
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Target: 5'- uCGCCGCGucaGGCGCGUCCugcGCCCcgggacgGCCGu -3' miRNA: 3'- uGCGGUGCc--CCGCGCGGG---CGGG-------CGGC- -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 13670 | 0.68 | 0.065895 |
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Target: 5'- uUGCCGCGGcauguacGGCgGCGCCCGCUCcauguCCGa -3' miRNA: 3'- uGCGGUGCC-------CCG-CGCGGGCGGGc----GGC- -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 13996 | 0.69 | 0.060877 |
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Target: 5'- uGCGcCCACGGaGGgccagaCGuCGCCuCGCUCGCCGc -3' miRNA: 3'- -UGC-GGUGCC-CC------GC-GCGG-GCGGGCGGC- -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 32463 | 1.07 | 0.000034 |
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Target: 5'- cACGCCACGGGGCGCGCCCGCCCGCCGg -3' miRNA: 3'- -UGCGGUGCCCCGCGCGGGCGGGCGGC- -5' |
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| 10660 | 5' | -69.6 | NC_002702.1 | + | 159 | 1.07 | 0.000034 |
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Target: 5'- cACGCCACGGGGCGCGCCCGCCCGCCGg -3' miRNA: 3'- -UGCGGUGCCCCGCGCGGGCGGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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