Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10666 | 5' | -53 | NC_002730.1 | + | 6202 | 0.66 | 0.70761 |
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Target: 5'- -gCAGggUGGCCU-GUUGcUGGCuGCc -3' miRNA: 3'- cgGUCuuACCGGAaCGACuAUCG-CGu -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 34056 | 0.66 | 0.684644 |
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Target: 5'- aGCCAuGAccUGGCUUUGUUGuUGGCGgAa -3' miRNA: 3'- -CGGU-CUu-ACCGGAACGACuAUCGCgU- -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 26756 | 0.67 | 0.626464 |
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Target: 5'- uGCCGGaAGUGGUCgUGUaucaAUGGCGCAu -3' miRNA: 3'- -CGGUC-UUACCGGaACGac--UAUCGCGU- -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 34756 | 0.67 | 0.614797 |
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Target: 5'- aGCCauAGAAUGGCCUUGCacGGUAaCGa- -3' miRNA: 3'- -CGG--UCUUACCGGAACGa-CUAUcGCgu -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 13575 | 0.67 | 0.603146 |
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Target: 5'- cGCCAGAGUucaccgcucGCCUUGUUGAccgcUGGCGa- -3' miRNA: 3'- -CGGUCUUAc--------CGGAACGACU----AUCGCgu -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 11045 | 0.67 | 0.601983 |
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Target: 5'- aGCCAGuaGAUGGCCUgacGCUuuucGAUgaacuggAGCGUAa -3' miRNA: 3'- -CGGUC--UUACCGGAa--CGA----CUA-------UCGCGU- -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 24430 | 0.69 | 0.530802 |
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Target: 5'- aGCCAGAgccggaaguacgugGUGGCggUGCUGAU-GCGa- -3' miRNA: 3'- -CGGUCU--------------UACCGgaACGACUAuCGCgu -5' |
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| 10666 | 5' | -53 | NC_002730.1 | + | 9355 | 1.1 | 0.000666 |
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Target: 5'- uGCCAGAAUGGCCUUGCUGAUAGCGCAc -3' miRNA: 3'- -CGGUCUUACCGGAACGACUAUCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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