Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10672 | 3' | -49 | NC_002730.1 | + | 30604 | 0.66 | 0.916923 |
Target: 5'- uGCAacGUCAGCCAGAAUGUCucagGCUaaGAg -3' miRNA: 3'- -CGUa-CGGUCGGUUUUAUAG----UGGgaCU- -5' |
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10672 | 3' | -49 | NC_002730.1 | + | 25552 | 0.67 | 0.870911 |
Target: 5'- gGCAcUGCUAGCCAGAcaAUGaaGCCCa-- -3' miRNA: 3'- -CGU-ACGGUCGGUUU--UAUagUGGGacu -5' |
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10672 | 3' | -49 | NC_002730.1 | + | 32871 | 0.71 | 0.668506 |
Target: 5'- cUAUGCCAGCCAuuAg--CACuCUUGAg -3' miRNA: 3'- cGUACGGUCGGUuuUauaGUG-GGACU- -5' |
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10672 | 3' | -49 | NC_002730.1 | + | 29853 | 0.73 | 0.53805 |
Target: 5'- -gAUGCCAGCCAGAAaaccaccagaauUAaUACCCUGu -3' miRNA: 3'- cgUACGGUCGGUUUU------------AUaGUGGGACu -5' |
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10672 | 3' | -49 | NC_002730.1 | + | 32167 | 0.73 | 0.526501 |
Target: 5'- -gAUGCCugacgcaaaGGCCAGAAUGUCAaaCCUGAa -3' miRNA: 3'- cgUACGG---------UCGGUUUUAUAGUg-GGACU- -5' |
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10672 | 3' | -49 | NC_002730.1 | + | 32276 | 1.12 | 0.001458 |
Target: 5'- gGCAUGCCAGCCAAAAUAUCACCCUGAa -3' miRNA: 3'- -CGUACGGUCGGUUUUAUAGUGGGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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