Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10674 | 3' | -52.3 | NC_002730.1 | + | 10076 | 0.67 | 0.711213 |
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Target: 5'- aCC-GCugCUuuGAUGCGGuuGUUGUa -3' miRNA: 3'- aGGuCGugGAggCUAUGCCu-UAACGg -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 33808 | 0.68 | 0.641691 |
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Target: 5'- gUCCuGCACgUCCacgccaaguuuuuGAUGCaGGcGUUGCCa -3' miRNA: 3'- -AGGuCGUGgAGG-------------CUAUG-CCuUAACGG- -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 4449 | 0.71 | 0.507092 |
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Target: 5'- uUCCGGCAaUUCCGuauCGGAggUGCUg -3' miRNA: 3'- -AGGUCGUgGAGGCuauGCCUuaACGG- -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 34859 | 0.72 | 0.453944 |
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Target: 5'- gUCCAGCACCuUCUGGUGacucuauAAUUGCCc -3' miRNA: 3'- -AGGUCGUGG-AGGCUAUgcc----UUAACGG- -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 4488 | 0.76 | 0.262203 |
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Target: 5'- gCCAGCGCCUCUguauGGUGCGGA--UGaCCc -3' miRNA: 3'- aGGUCGUGGAGG----CUAUGCCUuaAC-GG- -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 17619 | 0.84 | 0.076193 |
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Target: 5'- cUCCAGCGCCUCCGGcAUGGGcaAUgaUGCCa -3' miRNA: 3'- -AGGUCGUGGAGGCUaUGCCU--UA--ACGG- -5' |
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| 10674 | 3' | -52.3 | NC_002730.1 | + | 4382 | 1.14 | 0.000584 |
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Target: 5'- aUCCAGCACCUCCGAUACGGAAUUGCCg -3' miRNA: 3'- -AGGUCGUGGAGGCUAUGCCUUAACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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