Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1068 | 3' | -56 | NC_000929.1 | + | 9461 | 0.68 | 0.418134 |
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Target: 5'- uUCuGCUCGUCAGUGGCcugauuuuuuaucgGUGguguuaCUGGCGc -3' miRNA: 3'- -GGuCGAGUAGUCACCGa-------------CAC------GACCGU- -5' |
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| 1068 | 3' | -56 | NC_000929.1 | + | 28252 | 1.11 | 0.000288 |
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Target: 5'- aCCAGCUCAUCAGUGGCUGUGCUGGCAg -3' miRNA: 3'- -GGUCGAGUAGUCACCGACACGACCGU- -5' |
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| 1068 | 3' | -56 | NC_000929.1 | + | 27178 | 0.71 | 0.256374 |
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Target: 5'- uCCAGUgccgcaGUCAGUGcCUG-GCUGGCAg -3' miRNA: 3'- -GGUCGag----UAGUCACcGACaCGACCGU- -5' |
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| 1068 | 3' | -56 | NC_000929.1 | + | 14331 | 0.69 | 0.349622 |
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Target: 5'- gCAGgUCAUCAaguGUGGuCUGaUGCcUGGCAa -3' miRNA: 3'- gGUCgAGUAGU---CACC-GAC-ACG-ACCGU- -5' |
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| 1068 | 3' | -56 | NC_000929.1 | + | 13052 | 0.67 | 0.433988 |
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Target: 5'- aCCGGUUCAUugCGG-GGCUGUGCUuccccgauaucGGUc -3' miRNA: 3'- -GGUCGAGUA--GUCaCCGACACGA-----------CCGu -5' |
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| 1068 | 3' | -56 | NC_000929.1 | + | 28213 | 0.66 | 0.485666 |
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Target: 5'- aCCAGCUCucCGGcGGCauUGCUGGUc -3' miRNA: 3'- -GGUCGAGuaGUCaCCGacACGACCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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