Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10681 | 3' | -48.4 | NC_002730.1 | + | 12846 | 0.66 | 0.944297 |
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Target: 5'- -uUUGCCcGGGCUUGaagGCGAAuACGUc -3' miRNA: 3'- guAACGGcCCUGAAUga-CGUUU-UGCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 17204 | 0.66 | 0.944297 |
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Target: 5'- uCAUUGCCGGGA---GCUGguuAugGUc -3' miRNA: 3'- -GUAACGGCCCUgaaUGACguuUugCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 15207 | 0.66 | 0.938926 |
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Target: 5'- --gUGCCGcGACgccggGC-GCAAGAUGCa -3' miRNA: 3'- guaACGGCcCUGaa---UGaCGUUUUGCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 4842 | 0.66 | 0.920875 |
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Target: 5'- --gUGCCGGGAUUg---GaCAAuuAACGCa -3' miRNA: 3'- guaACGGCCCUGAaugaC-GUU--UUGCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 2989 | 0.66 | 0.920223 |
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Target: 5'- gCAUUGCagaacuggaggauCGGGAaaUACUGCucccAGAACGUa -3' miRNA: 3'- -GUAACG-------------GCCCUgaAUGACG----UUUUGCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 4118 | 0.67 | 0.907224 |
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Target: 5'- --aUGCCGGGAUUguaUGCAAguccucucAugGUa -3' miRNA: 3'- guaACGGCCCUGAaugACGUU--------UugCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 586 | 0.69 | 0.820863 |
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Target: 5'- --aUGCCGGGAuCUgguCUGCuggaaGAAACGg -3' miRNA: 3'- guaACGGCCCU-GAau-GACG-----UUUUGCg -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 27291 | 0.7 | 0.746065 |
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Target: 5'- uGUUGCCGGa--UUGCUGauGAACGCa -3' miRNA: 3'- gUAACGGCCcugAAUGACguUUUGCG- -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 29659 | 0.72 | 0.640845 |
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Target: 5'- cUAUUGCUGGGGCUggACUuGCGAAAUu- -3' miRNA: 3'- -GUAACGGCCCUGAa-UGA-CGUUUUGcg -5' |
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| 10681 | 3' | -48.4 | NC_002730.1 | + | 27186 | 1.12 | 0.002034 |
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Target: 5'- gCAUUGCCGGGACUUACUGCAAAACGCc -3' miRNA: 3'- -GUAACGGCCCUGAAUGACGUUUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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