Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10685 | 5' | -44.4 | NC_002766.1 | + | 3381 | 0.66 | 0.645437 |
Target: 5'- -aCCAACCACGAuAUUAuuUUCuGGAc -3' miRNA: 3'- caGGUUGGUGUUuUGAUu-AAGuCCUc -5' |
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10685 | 5' | -44.4 | NC_002766.1 | + | 3062 | 0.7 | 0.452346 |
Target: 5'- aUgCAGCCAUAAcuCUGAUaCGGGAGu -3' miRNA: 3'- cAgGUUGGUGUUuuGAUUAaGUCCUC- -5' |
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10685 | 5' | -44.4 | NC_002766.1 | + | 2576 | 0.7 | 0.402803 |
Target: 5'- uUCCAGCUugGAGACU--UUguGGGGu -3' miRNA: 3'- cAGGUUGGugUUUUGAuuAAguCCUC- -5' |
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10685 | 5' | -44.4 | NC_002766.1 | + | 3383 | 1.12 | 0.000407 |
Target: 5'- aGUCCAACCACAAAACUAAUUCAGGAGg -3' miRNA: 3'- -CAGGUUGGUGUUUUGAUUAAGUCCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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