Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10689 | 3' | -59.5 | NC_002786.1 | + | 1522 | 0.66 | 0.092649 |
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Target: 5'- cUCCGCCguccUGGGUcGCCuacucagccuuGGCCCUagcaGCCg -3' miRNA: 3'- -AGGCGGaa--GCCCAaUGG-----------CUGGGG----CGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 288 | 0.66 | 0.086847 |
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Target: 5'- cUCUGgCUUCGGccaUGCCGcucacccccACCCCGUCc -3' miRNA: 3'- -AGGCgGAAGCCca-AUGGC---------UGGGGCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 1957 | 0.67 | 0.081391 |
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Target: 5'- cUCCaGCgCUUCuGGgcGCCGACCUuCGCUu -3' miRNA: 3'- -AGG-CG-GAAGcCCaaUGGCUGGG-GCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 6070 | 0.67 | 0.081391 |
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Target: 5'- cCCGCCgucacuggUGGGgacACCaaGAUCCCGCUc -3' miRNA: 3'- aGGCGGaa------GCCCaa-UGG--CUGGGGCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 904 | 0.67 | 0.073818 |
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Target: 5'- uUCCGCgUUCcGGacGCCGucgcccuCCCCGCUc -3' miRNA: 3'- -AGGCGgAAGcCCaaUGGCu------GGGGCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 2060 | 0.69 | 0.048151 |
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Target: 5'- aCCGUCUUUGcGGUcacccCCGGCCCuCGCg -3' miRNA: 3'- aGGCGGAAGC-CCAau---GGCUGGG-GCGg -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 685 | 0.71 | 0.031259 |
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Target: 5'- uUCCGUUUUCaGGGUU-CUGACCuuGUCu -3' miRNA: 3'- -AGGCGGAAG-CCCAAuGGCUGGggCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 1415 | 0.71 | 0.031259 |
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Target: 5'- cCCGCCagUGcucuUUGCCGACUCCGCCu -3' miRNA: 3'- aGGCGGaaGCcc--AAUGGCUGGGGCGG- -5' |
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| 10689 | 3' | -59.5 | NC_002786.1 | + | 1017 | 1.12 | 0.000009 |
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Target: 5'- gUCCGCCUUCGGGUUACCGACCCCGCCg -3' miRNA: 3'- -AGGCGGAAGCCCAAUGGCUGGGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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