Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10698 | 3' | -55.5 | NC_002794.1 | + | 80025 | 0.7 | 0.783815 |
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Target: 5'- aGGCCGGGGAgaccaCUCGccgacagUGCcgGCGCGAGCc -3' miRNA: 3'- -CUGGUCCCU-----GAGC-------AUGa-CGCGCUUGc -5' |
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| 10698 | 3' | -55.5 | NC_002794.1 | + | 145905 | 0.71 | 0.75705 |
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Target: 5'- cGACgGGGGAgUCG-ACcGCGcCGAGCGg -3' miRNA: 3'- -CUGgUCCCUgAGCaUGaCGC-GCUUGC- -5' |
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| 10698 | 3' | -55.5 | NC_002794.1 | + | 102683 | 0.71 | 0.747603 |
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Target: 5'- cGACC-GGGugUCGUAC-GUGCuGACGg -3' miRNA: 3'- -CUGGuCCCugAGCAUGaCGCGcUUGC- -5' |
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| 10698 | 3' | -55.5 | NC_002794.1 | + | 153304 | 0.71 | 0.738058 |
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Target: 5'- aGGCCGGGGAgcggcugguCUCGUAUUacGUGCaGAACGg -3' miRNA: 3'- -CUGGUCCCU---------GAGCAUGA--CGCG-CUUGC- -5' |
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| 10698 | 3' | -55.5 | NC_002794.1 | + | 98939 | 0.75 | 0.520128 |
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Target: 5'- cGGCCGggaaggggcuGGGGCUCGUGCUGgGCGccgucGGCGg -3' miRNA: 3'- -CUGGU----------CCCUGAGCAUGACgCGC-----UUGC- -5' |
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| 10698 | 3' | -55.5 | NC_002794.1 | + | 195365 | 1.09 | 0.004539 |
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Target: 5'- gGACCAGGGACUCGUACUGCGCGAACGa -3' miRNA: 3'- -CUGGUCCCUGAGCAUGACGCGCUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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