Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10700 | 5' | -59 | NC_002794.1 | + | 36350 | 0.66 | 0.834302 |
Target: 5'- ---aCCcGG-CGUGCgcgGCCACCGGGUGc -3' miRNA: 3'- cacaGGuCCaGCACG---CGGUGGUCCAU- -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 3803 | 0.66 | 0.809295 |
Target: 5'- -gGUCCGGGUCG-GUGCCAaCCGc--- -3' miRNA: 3'- caCAGGUCCAGCaCGCGGU-GGUccau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 139402 | 0.66 | 0.800643 |
Target: 5'- uUGaCCAGGUCGUGCGCgAagaagaugaugCAGGUc -3' miRNA: 3'- cACaGGUCCAGCACGCGgUg----------GUCCAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 99430 | 0.67 | 0.782913 |
Target: 5'- -gGUCCGGGUuuguuggacCGgaucCGCCGCCGGGg- -3' miRNA: 3'- caCAGGUCCA---------GCac--GCGGUGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 182028 | 0.67 | 0.77385 |
Target: 5'- -cGUCCGGGcCGccgGCGaCCagACCAGGUc -3' miRNA: 3'- caCAGGUCCaGCa--CGC-GG--UGGUCCAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 57998 | 0.67 | 0.77385 |
Target: 5'- -cG-CCAGGccuUCGU-CGCCGCCGGGg- -3' miRNA: 3'- caCaGGUCC---AGCAcGCGGUGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 158743 | 0.67 | 0.745973 |
Target: 5'- -gGcCCGGGUagUGUGCGCgCACguGGUGa -3' miRNA: 3'- caCaGGUCCA--GCACGCG-GUGguCCAU- -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 70630 | 0.67 | 0.73648 |
Target: 5'- -cGcCCcGGUCGcacCGCCACCAGGUu -3' miRNA: 3'- caCaGGuCCAGCac-GCGGUGGUCCAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 67205 | 0.68 | 0.707515 |
Target: 5'- -cGUCCGGG-CgGUGCGCUcggcGCCGGGc- -3' miRNA: 3'- caCAGGUCCaG-CACGCGG----UGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 67310 | 0.68 | 0.697727 |
Target: 5'- ---cCCAGGUCGggucGCGCCcguacaucGCCAGGg- -3' miRNA: 3'- cacaGGUCCAGCa---CGCGG--------UGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 53592 | 0.68 | 0.678003 |
Target: 5'- cGUGUCgGcGUCG-GCGUCACCGGGc- -3' miRNA: 3'- -CACAGgUcCAGCaCGCGGUGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 135091 | 0.69 | 0.668083 |
Target: 5'- --cUCCAGG-CGUGCGCUggaGCUAGGa- -3' miRNA: 3'- cacAGGUCCaGCACGCGG---UGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 146977 | 0.69 | 0.658136 |
Target: 5'- cUGUCgCgAGGUCG-GCGCCGCCGGc-- -3' miRNA: 3'- cACAG-G-UCCAGCaCGCGGUGGUCcau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 21564 | 0.69 | 0.648168 |
Target: 5'- -aGUCCuccucGGGuucgUCGUGCGCgACCGGGg- -3' miRNA: 3'- caCAGG-----UCC----AGCACGCGgUGGUCCau -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 190650 | 0.7 | 0.618223 |
Target: 5'- cGUGUgCAGGaucaCGUcgcgcccgccGCGCCGCCGGGUc -3' miRNA: 3'- -CACAgGUCCa---GCA----------CGCGGUGGUCCAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 186227 | 0.7 | 0.588367 |
Target: 5'- -gGUCCGGGUCc--CGCCACCAGuGUc -3' miRNA: 3'- caCAGGUCCAGcacGCGGUGGUC-CAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 143021 | 0.7 | 0.578467 |
Target: 5'- cGUGcggucggCCGGGUCGacgUGCaggGCCGCCAGGUu -3' miRNA: 3'- -CACa------GGUCCAGC---ACG---CGGUGGUCCAu -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 69955 | 0.7 | 0.567619 |
Target: 5'- gGUGcCCGGGUUGUGCGUCACgcccagauacccgUGGGUGu -3' miRNA: 3'- -CACaGGUCCAGCACGCGGUG-------------GUCCAU- -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 195009 | 0.71 | 0.507664 |
Target: 5'- cUG-CCGGGcgaacuccucgaagUCGcGCGCCGCCAGGUGc -3' miRNA: 3'- cACaGGUCC--------------AGCaCGCGGUGGUCCAU- -5' |
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10700 | 5' | -59 | NC_002794.1 | + | 188946 | 0.72 | 0.46406 |
Target: 5'- cUGUCCGGG-CGUGCGCagguUCAGGUc -3' miRNA: 3'- cACAGGUCCaGCACGCGgu--GGUCCAu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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