Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10703 | 3' | -61.4 | NC_002794.1 | + | 180698 | 0.67 | 0.676858 |
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Target: 5'- --cGCCCGgcGGGCGgaCCAuGGCUCGGGu -3' miRNA: 3'- caaCGGGC--CCCGCg-GGU-CUGAGUCUg -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 182013 | 0.67 | 0.627947 |
|
Target: 5'- --aGCCCGGgaccccGGCGUCCGGGCcgCcGGCg -3' miRNA: 3'- caaCGGGCC------CCGCGGGUCUGa-GuCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 183728 | 0.66 | 0.705861 |
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Target: 5'- --aGCCCGcacGCGCCCAGcaggaUCAGGCc -3' miRNA: 3'- caaCGGGCcc-CGCGGGUCug---AGUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 184485 | 0.72 | 0.358817 |
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Target: 5'- --cGCgCGGGGC-CCCGGACgccCGGGCg -3' miRNA: 3'- caaCGgGCCCCGcGGGUCUGa--GUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 186015 | 0.68 | 0.588824 |
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Target: 5'- --cGCuCCGGGcccGgGCCCGGACcCGGGCc -3' miRNA: 3'- caaCG-GGCCC---CgCGGGUCUGaGUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 188946 | 0.67 | 0.657342 |
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Target: 5'- -cUGUCCGGGcGUGCgCAGGUUCAGGu -3' miRNA: 3'- caACGGGCCC-CGCGgGUCUGAGUCUg -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 191048 | 1.09 | 0.001075 |
|
Target: 5'- aGUUGCCCGGGGCGCCCAGACUCAGACa -3' miRNA: 3'- -CAACGGGCCCCGCGGGUCUGAGUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 195123 | 0.67 | 0.627947 |
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Target: 5'- --cGCCCGcGGcGCGCgCGGGCUCgcguacaccaccGGACc -3' miRNA: 3'- caaCGGGC-CC-CGCGgGUCUGAG------------UCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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