Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10703 | 3' | -61.4 | NC_002794.1 | + | 86515 | 0.68 | 0.588824 |
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Target: 5'- -aUGCuCCGGGccGCGCCC-GAC-CGGAUg -3' miRNA: 3'- caACG-GGCCC--CGCGGGuCUGaGUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 182013 | 0.67 | 0.627947 |
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Target: 5'- --aGCCCGGgaccccGGCGUCCGGGCcgCcGGCg -3' miRNA: 3'- caaCGGGCC------CCGCGGGUCUGa-GuCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 141441 | 0.68 | 0.588824 |
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Target: 5'- --cGCCgGGGGCGCCgGGAg-CGGcGCg -3' miRNA: 3'- caaCGGgCCCCGCGGgUCUgaGUC-UG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 82188 | 0.67 | 0.637752 |
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Target: 5'- --aGCCCGGGcgccccgaGCGCCCGGcACUCc--- -3' miRNA: 3'- caaCGGGCCC--------CGCGGGUC-UGAGucug -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 195123 | 0.67 | 0.627947 |
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Target: 5'- --cGCCCGcGGcGCGCgCGGGCUCgcguacaccaccGGACc -3' miRNA: 3'- caaCGGGC-CC-CGCGgGUCUGAG------------UCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 86563 | 0.67 | 0.647553 |
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Target: 5'- -aUGCuCCGGGccGCGCCC-GAC-CGGAUu -3' miRNA: 3'- caACG-GGCCC--CGCGGGuCUGaGUCUG- -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 188946 | 0.67 | 0.657342 |
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Target: 5'- -cUGUCCGGGcGUGCgCAGGUUCAGGu -3' miRNA: 3'- caACGGGCCC-CGCGgGUCUGAGUCUg -5' |
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| 10703 | 3' | -61.4 | NC_002794.1 | + | 148134 | 0.66 | 0.734348 |
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Target: 5'- --gGCgCGGGGcCGCCgGGGCgaGGGCg -3' miRNA: 3'- caaCGgGCCCC-GCGGgUCUGagUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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