Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10703 | 5' | -54 | NC_002794.1 | + | 128681 | 0.73 | 0.74412 |
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Target: 5'- aUCGCCgcggCGGUCCGGAggcuCG-GAACAGAc -3' miRNA: 3'- -GGCGG----GCCAGGUCUau--GCaCUUGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 77782 | 0.74 | 0.705269 |
|
Target: 5'- cCCGCaCGGUCCAGAU-CGUGcAGcCGGAg -3' miRNA: 3'- -GGCGgGCCAGGUCUAuGCAC-UU-GUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 48566 | 0.74 | 0.715093 |
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Target: 5'- gCCGCCC-GUCCAGAUGgGcGAGcCGGAg -3' miRNA: 3'- -GGCGGGcCAGGUCUAUgCaCUU-GUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 149343 | 0.75 | 0.635298 |
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Target: 5'- aCCGgCCGGggUCgCGGggGCGUGGACGGAg -3' miRNA: 3'- -GGCgGGCC--AG-GUCuaUGCACUUGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 127685 | 0.76 | 0.605114 |
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Target: 5'- -aGCCCGGUCgGGGcgACGUGggUGGGa -3' miRNA: 3'- ggCGGGCCAGgUCUa-UGCACuuGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 191083 | 1.11 | 0.005396 |
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Target: 5'- gCCGCCCGGUCCAGAUACGUGAACAGAc -3' miRNA: 3'- -GGCGGGCCAGGUCUAUGCACUUGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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