Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10703 | 5' | -54 | NC_002794.1 | + | 115611 | 0.66 | 0.978865 |
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Target: 5'- cUCGCCCGGcgCCGGGagGCG-GGuCAGGu -3' miRNA: 3'- -GGCGGGCCa-GGUCUa-UGCaCUuGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 30394 | 0.66 | 0.98101 |
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Target: 5'- gCCGCCCGcGaCguGAUGcCGUGGucgaacgucguGCAGAu -3' miRNA: 3'- -GGCGGGC-CaGguCUAU-GCACU-----------UGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 35657 | 0.66 | 0.982988 |
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Target: 5'- gCGCCCGGUCUccg-ACGUGGuCAa- -3' miRNA: 3'- gGCGGGCCAGGucuaUGCACUuGUcu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 88460 | 0.66 | 0.982988 |
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Target: 5'- aUGCCUGGUCCgcAGAUAcCGUugGAGCGu- -3' miRNA: 3'- gGCGGGCCAGG--UCUAU-GCA--CUUGUcu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 33079 | 0.66 | 0.984277 |
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Target: 5'- aCGCUCGaauucuUCCAGAUccuccacggcgugaACGUGAGCGGc -3' miRNA: 3'- gGCGGGCc-----AGGUCUA--------------UGCACUUGUCu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 183411 | 0.66 | 0.984806 |
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Target: 5'- -aGCCaCGGgaCCAGAaGCGgGAGCGGGa -3' miRNA: 3'- ggCGG-GCCa-GGUCUaUGCaCUUGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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