Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10703 | 5' | -54 | NC_002794.1 | + | 44453 | 0.67 | 0.969648 |
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Target: 5'- cCCGCCCGGacgUCGGcgGCG-GAgaccgccccgucgaaACAGAg -3' miRNA: 3'- -GGCGGGCCa--GGUCuaUGCaCU---------------UGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 45943 | 0.67 | 0.971355 |
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Target: 5'- cCCGCCCGGUgaccgucucUCGGGcGCGUGAu---- -3' miRNA: 3'- -GGCGGGCCA---------GGUCUaUGCACUugucu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 192998 | 0.67 | 0.971355 |
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Target: 5'- cUCGCCCGGcgugugcagcgCCAGcgGCGUGuACAu- -3' miRNA: 3'- -GGCGGGCCa----------GGUCuaUGCACuUGUcu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 133934 | 0.67 | 0.971355 |
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Target: 5'- gCGCCC-GUCCAGuUGCGgggUGAcCGGAa -3' miRNA: 3'- gGCGGGcCAGGUCuAUGC---ACUuGUCU- -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 47740 | 0.67 | 0.971355 |
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Target: 5'- cCCGCCgGGUCCAcGUGgcggcCGUGGucGCGGc -3' miRNA: 3'- -GGCGGgCCAGGUcUAU-----GCACU--UGUCu -5' |
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| 10703 | 5' | -54 | NC_002794.1 | + | 33079 | 0.66 | 0.984277 |
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Target: 5'- aCGCUCGaauucuUCCAGAUccuccacggcgugaACGUGAGCGGc -3' miRNA: 3'- gGCGGGCc-----AGGUCUA--------------UGCACUUGUCu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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