Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10706 | 3' | -56.5 | NC_002794.1 | + | 129208 | 0.66 | 0.903075 |
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Target: 5'- cGAGCgGCCGA--CGGCGGUggauaGGCGCg -3' miRNA: 3'- -UUUG-CGGCUagGCCGUCAag---CCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 120069 | 0.66 | 0.903075 |
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Target: 5'- cGGAgGCCGAcgCGGCGGUggCGGCGa- -3' miRNA: 3'- -UUUgCGGCUagGCCGUCAa-GCCGUga -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 49768 | 0.66 | 0.908587 |
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Target: 5'- -cGCGCUcGUCUccccggaGGCGGUUgGGCACg -3' miRNA: 3'- uuUGCGGcUAGG-------CCGUCAAgCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 57407 | 0.66 | 0.909188 |
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Target: 5'- cGGCGCCcgcguggCCGGCGugcuggUCGGCACg -3' miRNA: 3'- uUUGCGGcua----GGCCGUca----AGCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 111317 | 0.66 | 0.909188 |
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Target: 5'- gAGACGCCGggCCaGCGGcgCGGgcCGCa -3' miRNA: 3'- -UUUGCGGCuaGGcCGUCaaGCC--GUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 191659 | 0.66 | 0.909188 |
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Target: 5'- --cCGCCGGgggacggcgCCGGCAGcggCGGCGg- -3' miRNA: 3'- uuuGCGGCUa--------GGCCGUCaa-GCCGUga -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 181227 | 0.66 | 0.915069 |
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Target: 5'- cGACGa-GAUCCGGCGa--CGGCGCg -3' miRNA: 3'- uUUGCggCUAGGCCGUcaaGCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 73058 | 0.66 | 0.896735 |
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Target: 5'- -cACGCUGAacuUCUGGCAGgaggacgGGCGCa -3' miRNA: 3'- uuUGCGGCU---AGGCCGUCaag----CCGUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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