Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10706 | 3' | -56.5 | NC_002794.1 | + | 32010 | 0.66 | 0.920718 |
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Target: 5'- cGACGCaGA-CCaGGCAGgggacgCGGCGCUg -3' miRNA: 3'- uUUGCGgCUaGG-CCGUCaa----GCCGUGA- -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 133381 | 0.66 | 0.920718 |
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Target: 5'- --uCGUCG-UCCGGCGGUcaugaUCGGCcCg -3' miRNA: 3'- uuuGCGGCuAGGCCGUCA-----AGCCGuGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 101551 | 0.66 | 0.926133 |
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Target: 5'- cGAACGCCGuuccgaacgCCGGCGGgagugcCGGC-Cg -3' miRNA: 3'- -UUUGCGGCua-------GGCCGUCaa----GCCGuGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 104743 | 0.66 | 0.926133 |
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Target: 5'- gGAGCGCuCGAUCUcguGGUAGcggUCGGCcCg -3' miRNA: 3'- -UUUGCG-GCUAGG---CCGUCa--AGCCGuGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 65986 | 0.66 | 0.926133 |
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Target: 5'- cAACGUCGAaCUgGGCGGUgauccCGGCACc -3' miRNA: 3'- uUUGCGGCUaGG-CCGUCAa----GCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 90082 | 0.66 | 0.926133 |
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Target: 5'- ---gGCCGAUgUGGC---UCGGCACg -3' miRNA: 3'- uuugCGGCUAgGCCGucaAGCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 34745 | 0.66 | 0.926133 |
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Target: 5'- aGAGCGCgCGAcggCgCGGUGGgacgCGGCACg -3' miRNA: 3'- -UUUGCG-GCUa--G-GCCGUCaa--GCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 102037 | 0.66 | 0.926133 |
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Target: 5'- --uCGCCGAgCCGGC-GUuccUCGcGCGCUu -3' miRNA: 3'- uuuGCGGCUaGGCCGuCA---AGC-CGUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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