Results 81 - 88 of 88 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10706 | 3' | -56.5 | NC_002794.1 | + | 34745 | 0.66 | 0.926133 |
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Target: 5'- aGAGCGCgCGAcggCgCGGUGGgacgCGGCACg -3' miRNA: 3'- -UUUGCG-GCUa--G-GCCGUCaa--GCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 32859 | 0.69 | 0.75875 |
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Target: 5'- cAGCgGCCGAgCCGGCGGgcggCGGCGg- -3' miRNA: 3'- uUUG-CGGCUaGGCCGUCaa--GCCGUga -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 32010 | 0.66 | 0.920718 |
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Target: 5'- cGACGCaGA-CCaGGCAGgggacgCGGCGCUg -3' miRNA: 3'- uUUGCGgCUaGG-CCGUCaa----GCCGUGA- -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 29203 | 0.67 | 0.869153 |
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Target: 5'- --cCGUCcGUCCGGCGG-UCGGCGg- -3' miRNA: 3'- uuuGCGGcUAGGCCGUCaAGCCGUga -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 22719 | 0.66 | 0.896735 |
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Target: 5'- cGGCGCCGAgagCCGGCGaacggUCGGUc-- -3' miRNA: 3'- uUUGCGGCUa--GGCCGUca---AGCCGuga -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 20748 | 0.66 | 0.903075 |
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Target: 5'- -cGCGCUGAUCCGcCAGcucucggaaCGGCGCg -3' miRNA: 3'- uuUGCGGCUAGGCcGUCaa-------GCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 17500 | 0.71 | 0.690472 |
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Target: 5'- --uCGCCGAcgCCGGCcgAGaUCGGCGCc -3' miRNA: 3'- uuuGCGGCUa-GGCCG--UCaAGCCGUGa -5' |
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| 10706 | 3' | -56.5 | NC_002794.1 | + | 12199 | 0.7 | 0.739656 |
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Target: 5'- cGGGCGCCGcucggacgugCCGGCGGcgUCGGCGg- -3' miRNA: 3'- -UUUGCGGCua--------GGCCGUCa-AGCCGUga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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