Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1071 | 5' | -58.1 | NC_000929.1 | + | 5640 | 0.68 | 0.362295 |
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Target: 5'- aGGGCGGUUGUGGcGACUuUCAguuuuuCCgCCAu -3' miRNA: 3'- -CCCGCCAGCGCCaUUGAcAGU------GG-GGU- -5' |
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| 1071 | 5' | -58.1 | NC_000929.1 | + | 11024 | 0.68 | 0.353754 |
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Target: 5'- cGGGgGGUUGUGGcacaGGCUGgacagCACCCg- -3' miRNA: 3'- -CCCgCCAGCGCCa---UUGACa----GUGGGgu -5' |
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| 1071 | 5' | -58.1 | NC_000929.1 | + | 25410 | 0.7 | 0.258504 |
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Target: 5'- cGGCGGUCgucguguacggagcgGCGGaAgaauggccccGCUGUUACCCCGc -3' miRNA: 3'- cCCGCCAG---------------CGCCaU----------UGACAGUGGGGU- -5' |
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| 1071 | 5' | -58.1 | NC_000929.1 | + | 19022 | 0.75 | 0.119716 |
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Target: 5'- cGGCGGUgGCGGU-GCUGUCugCUg- -3' miRNA: 3'- cCCGCCAgCGCCAuUGACAGugGGgu -5' |
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| 1071 | 5' | -58.1 | NC_000929.1 | + | 29612 | 1.1 | 0.000306 |
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Target: 5'- gGGGCGGUCGCGGUAACUGUCACCCCAc -3' miRNA: 3'- -CCCGCCAGCGCCAUUGACAGUGGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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