miRNA: 3'- -GAG-GC-UCCGCuuCGAGCAGAGGcuuc -5'
miRNA: 3'- -GAGGCUCCG----CUUC-GAG-------CAGAGGCuuC- -5'
miRNA: 3'- -GAGGCUCcGCUUCGAGCAGAGgCUUc -5'
miRNA: 3'- -GAGGCUCCGCUUCGAGCAGAGGCUUC- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10715 | 3' | -55.7 | NC_002794.1 | + | 112662 | 0.72 | 0.717973 |
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Target: 5'- gCUCuCGcAGGCGgcGUUCGUCUCCa--- -3' miRNA: 3'- -GAG-GC-UCCGCuuCGAGCAGAGGcuuc -5' |
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| 10715 | 3' | -55.7 | NC_002794.1 | + | 41319 | 0.74 | 0.587666 |
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Target: 5'- uCUCCGAGGUcuccGAGGuCUCcuccgggGUCUCCGggGu -3' miRNA: 3'- -GAGGCUCCG----CUUC-GAG-------CAGAGGCuuC- -5' |
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| 10715 | 3' | -55.7 | NC_002794.1 | + | 181043 | 0.84 | 0.167256 |
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Target: 5'- uCUCCGAGaCGAAGCUCGUCUCgGAGa -3' miRNA: 3'- -GAGGCUCcGCUUCGAGCAGAGgCUUc -5' |
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| 10715 | 3' | -55.7 | NC_002794.1 | + | 181007 | 1.08 | 0.005151 |
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Target: 5'- cCUCCGAGGCGAAGCUCGUCUCCGAAGc -3' miRNA: 3'- -GAGGCUCCGCUUCGAGCAGAGGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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