Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10716 | 5' | -57.8 | NC_002794.1 | + | 113361 | 0.66 | 0.900495 |
Target: 5'- gGCgUUUCCCggaACGGGUGCGggccgcCGUCGu -3' miRNA: 3'- -UGaGGAGGGag-UGCCCACGUa-----GCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 138865 | 0.67 | 0.852151 |
Target: 5'- gACUCuCggCCUCGCGGGa--GUCGUCGc -3' miRNA: 3'- -UGAG-GagGGAGUGCCCacgUAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 62671 | 0.69 | 0.749497 |
Target: 5'- cCUCCUCgagaaccgCCU-GCGGGUGCAgcugUGUCGg -3' miRNA: 3'- uGAGGAG--------GGAgUGCCCACGUa---GCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 146227 | 0.7 | 0.711511 |
Target: 5'- -gUCCUCCUcggUCGCGGcGUcGuCAUCGUCGu -3' miRNA: 3'- ugAGGAGGG---AGUGCC-CA-C-GUAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 91031 | 0.7 | 0.681349 |
Target: 5'- aGCUCUgggcggCCCUCgggguggACGGGgcgcGCGUCGUCa -3' miRNA: 3'- -UGAGGa-----GGGAG-------UGCCCa---CGUAGCAGc -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 21750 | 0.71 | 0.646869 |
Target: 5'- uGCgCCUCCCUcCACGGGaucgagaagccacgGCuUCGUCGg -3' miRNA: 3'- -UGaGGAGGGA-GUGCCCa-------------CGuAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 90529 | 0.71 | 0.623128 |
Target: 5'- cGCUCgUCUC-CGCGGGUGaCGUCGUg- -3' miRNA: 3'- -UGAGgAGGGaGUGCCCAC-GUAGCAgc -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 195019 | 0.72 | 0.564138 |
Target: 5'- aACUCCUCgaagUCGCGcgccgccaGGUGCAUCGUCGu -3' miRNA: 3'- -UGAGGAGgg--AGUGC--------CCACGUAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 6721 | 0.75 | 0.39973 |
Target: 5'- uGCUCUgCCCUCaggaaauGCGGGagaUGCAUCGUCGc -3' miRNA: 3'- -UGAGGaGGGAG-------UGCCC---ACGUAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 131606 | 0.77 | 0.328676 |
Target: 5'- gACUCCUCCCcugaucaccaccucUCcCGGGaGCGUCGUCGc -3' miRNA: 3'- -UGAGGAGGG--------------AGuGCCCaCGUAGCAGC- -5' |
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10716 | 5' | -57.8 | NC_002794.1 | + | 178386 | 1.07 | 0.003527 |
Target: 5'- gACUCCUCCCUCACGGGUGCAUCGUCGc -3' miRNA: 3'- -UGAGGAGGGAGUGCCCACGUAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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