Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10717 | 3' | -54 | NC_002794.1 | + | 66627 | 0.66 | 0.983416 |
Target: 5'- aGGcGGugGCGGGAGGaggucCGUccGGGGGCaUCg -3' miRNA: 3'- -CC-CCugUGCUCUCU-----GCAa-CUCCUG-AG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 116412 | 0.66 | 0.981467 |
Target: 5'- cGGGGCGCGAGcaccaGGGCGcgGgucAGGACg- -3' miRNA: 3'- cCCCUGUGCUC-----UCUGCaaC---UCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 115860 | 0.66 | 0.981467 |
Target: 5'- uGGGACGCGcGAGGgGUccGGGGCg- -3' miRNA: 3'- cCCCUGUGCuCUCUgCAacUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 107604 | 0.66 | 0.979353 |
Target: 5'- gGGGGAgACGGGgaAGACG---GGGACg- -3' miRNA: 3'- -CCCCUgUGCUC--UCUGCaacUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 156469 | 0.66 | 0.977064 |
Target: 5'- aGGGG--GCGGGGGACGacgGGGcGGCUUu -3' miRNA: 3'- -CCCCugUGCUCUCUGCaa-CUC-CUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 149462 | 0.66 | 0.974594 |
Target: 5'- aGGGGGCGguCGAGGaGCGaggGGGGGCg- -3' miRNA: 3'- -CCCCUGU--GCUCUcUGCaa-CUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 140039 | 0.66 | 0.974594 |
Target: 5'- cGGGcGGCGgGAGAcACGgaaaGGGGCUCa -3' miRNA: 3'- -CCC-CUGUgCUCUcUGCaac-UCCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 118994 | 0.67 | 0.971935 |
Target: 5'- -cGGGCGCgGGGAGACGagGGGGAa-- -3' miRNA: 3'- ccCCUGUG-CUCUCUGCaaCUCCUgag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 115887 | 0.67 | 0.962768 |
Target: 5'- cGGGGcguccgcggaGCGCGGGGGGuuCGUgggGuGGACUUg -3' miRNA: 3'- -CCCC----------UGUGCUCUCU--GCAa--CuCCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 110293 | 0.67 | 0.955607 |
Target: 5'- -cGGGCcCGAGAGcCGc-GGGGACUCu -3' miRNA: 3'- ccCCUGuGCUCUCuGCaaCUCCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 149349 | 0.69 | 0.923452 |
Target: 5'- cGGGGuCGCG-GGGGCGUgGAcGGAgUCc -3' miRNA: 3'- -CCCCuGUGCuCUCUGCAaCU-CCUgAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 124163 | 0.69 | 0.917932 |
Target: 5'- cGGGGCcCGGGAGAgCGUcggggaGGGGACUg -3' miRNA: 3'- cCCCUGuGCUCUCU-GCAa-----CUCCUGAg -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 127707 | 0.69 | 0.906197 |
Target: 5'- uGGGACAgGAGA-ACGaUG-GGGCUCg -3' miRNA: 3'- cCCCUGUgCUCUcUGCaACuCCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 126718 | 0.7 | 0.886886 |
Target: 5'- cGGGGCGagGGGGGugGgUGGGGACg- -3' miRNA: 3'- cCCCUGUg-CUCUCugCaACUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 141426 | 0.7 | 0.880006 |
Target: 5'- cGGGGGCgGCG-GGGGCGccGGGGGCg- -3' miRNA: 3'- -CCCCUG-UGCuCUCUGCaaCUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 136339 | 0.7 | 0.87291 |
Target: 5'- -cGGugGCGGGccagcguGGCGUUGAGG-CUCa -3' miRNA: 3'- ccCCugUGCUCu------CUGCAACUCCuGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 94061 | 0.71 | 0.858093 |
Target: 5'- cGGGGGCgGCGGGGGGC---GAGGGCa- -3' miRNA: 3'- -CCCCUG-UGCUCUCUGcaaCUCCUGag -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 126693 | 0.71 | 0.842481 |
Target: 5'- cGGGGAgG-GAGGGGCGccGcGGACUCg -3' miRNA: 3'- -CCCCUgUgCUCUCUGCaaCuCCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 40114 | 0.73 | 0.744934 |
Target: 5'- cGGGGGCGCGcGAGAgCGgaGAgccacGGGCUCu -3' miRNA: 3'- -CCCCUGUGCuCUCU-GCaaCU-----CCUGAG- -5' |
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10717 | 3' | -54 | NC_002794.1 | + | 149824 | 0.74 | 0.696054 |
Target: 5'- cGGGGACcCGAG-GACGggcGGGGACg- -3' miRNA: 3'- -CCCCUGuGCUCuCUGCaa-CUCCUGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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