miRNA: 3'- -CGCUUC--------UGGCG--AUUGCCUCa---------GGCUUu -5'
miRNA: 3'- -CGCuuCUgGCGaUUGCCucAGGCUUU- -5'
miRNA: 3'- cGCUUCU-----------GGCGauuGCCUCAG-GCUUu -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10719 | 5' | -52.2 | NC_002794.1 | + | 102189 | 0.66 | 0.988738 |
|
Target: 5'- gGCGAGGaccuggcgGCCGCcgUGACGGAGgcccucuucaCCGAGc -3' miRNA: 3'- -CGCUUC--------UGGCG--AUUGCCUCa---------GGCUUu -5' |
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| 10719 | 5' | -52.2 | NC_002794.1 | + | 55313 | 0.66 | 0.988738 |
|
Target: 5'- cGCGgcGGgCGCgGGCGGcuUCCGAGGa -3' miRNA: 3'- -CGCuuCUgGCGaUUGCCucAGGCUUU- -5' |
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| 10719 | 5' | -52.2 | NC_002794.1 | + | 81584 | 0.66 | 0.993394 |
|
Target: 5'- aCGAAGAagaagacggacCCGCggcCGGAGUCgCGGAc -3' miRNA: 3'- cGCUUCU-----------GGCGauuGCCUCAG-GCUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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