Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10722 | 3' | -52.9 | NC_002794.1 | + | 40311 | 0.69 | 0.939956 |
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Target: 5'- aGGAGGGACGGuuuUCCucacccgCCGCGCGGa- -3' miRNA: 3'- aCCUCUCUGCUuu-AGG-------GGUGUGCUca -5' |
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| 10722 | 3' | -52.9 | NC_002794.1 | + | 83054 | 0.7 | 0.919752 |
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Target: 5'- cGGAG-GACGAGcgCCCaCcCACGGGc -3' miRNA: 3'- aCCUCuCUGCUUuaGGG-GuGUGCUCa -5' |
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| 10722 | 3' | -52.9 | NC_002794.1 | + | 79459 | 0.7 | 0.901093 |
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Target: 5'- cGGAGAcGACGAcgccgccAGUCgCCAC-CGAGUu -3' miRNA: 3'- aCCUCU-CUGCU-------UUAGgGGUGuGCUCA- -5' |
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| 10722 | 3' | -52.9 | NC_002794.1 | + | 135654 | 0.72 | 0.835488 |
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Target: 5'- cGGAGGGugGGGggguGUCCuCCACgACGGGc -3' miRNA: 3'- aCCUCUCugCUU----UAGG-GGUG-UGCUCa -5' |
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| 10722 | 3' | -52.9 | NC_002794.1 | + | 155946 | 0.73 | 0.773389 |
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Target: 5'- cGGGGcucuuCGccGUCCCCACACGAGUc -3' miRNA: 3'- aCCUCucu--GCuuUAGGGGUGUGCUCA- -5' |
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| 10722 | 3' | -52.9 | NC_002794.1 | + | 159377 | 1.08 | 0.008234 |
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Target: 5'- gUGGAGAGACGAAAUCCCCACACGAGUg -3' miRNA: 3'- -ACCUCUCUGCUUUAGGGGUGUGCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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