miRNA: 3'- -ACCGCa--CGUGG-CUGUaGCaCUGCCac -5'
miRNA: 3'- aC-CGCACGUGGCUGUagcaCUGCCAc -5'
miRNA: 3'- aCCGC-ACGugGCUGUAGC--ACU-GCCAC- -5'
miRNA: 3'- -ACCGc---ACGuGGCUGuAGCa---CUGCCAC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10723 | 5' | -56.5 | NC_002794.1 | + | 14597 | 0.66 | 0.936882 |
|
Target: 5'- gUGGCGagcGCGCCgGGCAcCGcGACGGg- -3' miRNA: 3'- -ACCGCa--CGUGG-CUGUaGCaCUGCCac -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 9340 | 0.7 | 0.753667 |
|
Target: 5'- gGuGCGUGCGCCGGCAc---GACGGUc -3' miRNA: 3'- aC-CGCACGUGGCUGUagcaCUGCCAc -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 4199 | 0.71 | 0.68604 |
|
Target: 5'- aGGCGaUGCugUGACGUUGcaUGAcCGGUGu -3' miRNA: 3'- aCCGC-ACGugGCUGUAGC--ACU-GCCAC- -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 544 | 0.66 | 0.92165 |
|
Target: 5'- uUGGCaucuUGC-CCGGCcUCGgcgcGGCGGUGg -3' miRNA: 3'- -ACCGc---ACGuGGCUGuAGCa---CUGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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