miRNA: 3'- aCCGCACG---UGGCUGU------AGCa------CUGCCac -5'
miRNA: 3'- -ACCGc---ACGuGGCUGuAGCa---CUGCCAC- -5'
miRNA: 3'- aCCGCA-CguGGCUGuAGCaCUGCCac -5'
miRNA: 3'- aCCGCAC------------GUGGCUGUAG---------CACUGCcac -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10723 | 5' | -56.5 | NC_002794.1 | + | 155979 | 0.66 | 0.916118 |
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Target: 5'- gGGCGUGUcgaACCGACGacgaucUCGgacgagcGACGGg- -3' miRNA: 3'- aCCGCACG---UGGCUGU------AGCa------CUGCCac -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 544 | 0.66 | 0.92165 |
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Target: 5'- uUGGCaucuUGC-CCGGCcUCGgcgcGGCGGUGg -3' miRNA: 3'- -ACCGc---ACGuGGCUGuAGCa---CUGCCAC- -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 14663 | 0.66 | 0.926955 |
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Target: 5'- cGGCGUcGguCCGAC-UCGcGGCGGc- -3' miRNA: 3'- aCCGCA-CguGGCUGuAGCaCUGCCac -5' |
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| 10723 | 5' | -56.5 | NC_002794.1 | + | 159511 | 0.66 | 0.941505 |
|
Target: 5'- cGGCGUGggucgaaucauaUACCGACAUCaacggacuaGUGACGa-- -3' miRNA: 3'- aCCGCAC------------GUGGCUGUAG---------CACUGCcac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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