Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
10725 | 3' | -52.6 | NC_002794.1 | + | 136375 | 0.66 | 0.98759 |
Target: 5'- -aACAUGAGGUCGuug-AGcACCGGCUc -3' miRNA: 3'- ucUGUGCUCUAGCcuuaUC-UGGCCGA- -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 108468 | 0.66 | 0.991544 |
Target: 5'- cGGCACGGGc-CGaGGGgcGACCGGCc -3' miRNA: 3'- uCUGUGCUCuaGC-CUUauCUGGCCGa -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 86927 | 0.66 | 0.991544 |
Target: 5'- cGugGCGGGcaggCGGGGcgGGAuCCGGCg -3' miRNA: 3'- uCugUGCUCua--GCCUUa-UCU-GGCCGa -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 141446 | 0.66 | 0.991544 |
Target: 5'- gGGGCGcCGGGAgcggcgCGGcGUccGGACUGGCg -3' miRNA: 3'- -UCUGU-GCUCUa-----GCCuUA--UCUGGCCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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