Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10725 | 3' | -52.6 | NC_002794.1 | + | 183417 | 0.77 | 0.580578 |
Target: 5'- gGGACcagaaGCGGGAgCGGGAgcgGGACCGGCg -3' miRNA: 3'- -UCUG-----UGCUCUaGCCUUa--UCUGGCCGa -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 191288 | 0.68 | 0.963839 |
Target: 5'- gAGACAccacaccacCGAGAcaCGGAugcgagGGGCCGGCg -3' miRNA: 3'- -UCUGU---------GCUCUa-GCCUua----UCUGGCCGa -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 191846 | 0.72 | 0.858135 |
Target: 5'- gAGACGCGcaaucguGGUCGuGAA-AGGCCGGCg -3' miRNA: 3'- -UCUGUGCu------CUAGC-CUUaUCUGGCCGa -5' |
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10725 | 3' | -52.6 | NC_002794.1 | + | 191892 | 0.84 | 0.259677 |
Target: 5'- cGGAgGCGGGAUCGGcaccgGGACCGGCUg -3' miRNA: 3'- -UCUgUGCUCUAGCCuua--UCUGGCCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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