Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1073 | 5' | -53.6 | NC_000929.1 | + | 16586 | 0.71 | 0.42222 |
Target: 5'- aCCCGCccaauGGCuggaacUGCCGCuGUcgCGUCCg -3' miRNA: 3'- -GGGCG-----UCGuaa---ACGGCG-CGuaGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 17451 | 0.69 | 0.512823 |
Target: 5'- aCCGCGcCAUUccccgagugauggcUGCgGCGCugcuGUCAUCCa -3' miRNA: 3'- gGGCGUcGUAA--------------ACGgCGCG----UAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 15547 | 0.69 | 0.538136 |
Target: 5'- gCCCGCAGUAUUaaCCGUGaccugauUUAUCCc -3' miRNA: 3'- -GGGCGUCGUAAacGGCGCgu-----AGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 3026 | 0.67 | 0.605865 |
Target: 5'- gCUGCGGCAg--GCCGUGaaUAUCG-CCg -3' miRNA: 3'- gGGCGUCGUaaaCGGCGC--GUAGUaGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 20662 | 0.67 | 0.605865 |
Target: 5'- gCCGCGcGCuuuguucUGCCGCuuacCGUCGUCCc -3' miRNA: 3'- gGGCGU-CGuaa----ACGGCGc---GUAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 20264 | 0.66 | 0.719434 |
Target: 5'- gCCGaCAGCG--UGCUGUcugugguaucuGCAUCGUCa -3' miRNA: 3'- gGGC-GUCGUaaACGGCG-----------CGUAGUAGg -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 16059 | 0.71 | 0.42222 |
Target: 5'- gCCGCAGCAgaccaUUgaucuggcguaUGCCGCGCG-CcUCCc -3' miRNA: 3'- gGGCGUCGU-----AA-----------ACGGCGCGUaGuAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 33667 | 0.66 | 0.708293 |
Target: 5'- aCUCaCAGCuacgGCUGgGCAUCAuuUCCg -3' miRNA: 3'- -GGGcGUCGuaaaCGGCgCGUAGU--AGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 7443 | 0.66 | 0.697071 |
Target: 5'- -aUGCGGCc-UUGCgCGgGUGUCAUCCc -3' miRNA: 3'- ggGCGUCGuaAACG-GCgCGUAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 15036 | 0.66 | 0.674435 |
Target: 5'- -aUGCAGCGccaccgGCaCGCGCAUUuUCCc -3' miRNA: 3'- ggGCGUCGUaaa---CG-GCGCGUAGuAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 13898 | 0.66 | 0.663047 |
Target: 5'- gCCGUGGCGcaUUUGUgGUGUcagcgauucaaaAUCGUCCg -3' miRNA: 3'- gGGCGUCGU--AAACGgCGCG------------UAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 8769 | 0.67 | 0.651627 |
Target: 5'- gCCGUcauGGCAUcauaGCCGCGCAguUCAcgaCCg -3' miRNA: 3'- gGGCG---UCGUAaa--CGGCGCGU--AGUa--GG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 28506 | 0.67 | 0.651627 |
Target: 5'- gUCUGCGGCG-UUGCUcacgcaggGCGCGggccugugCAUCCu -3' miRNA: 3'- -GGGCGUCGUaAACGG--------CGCGUa-------GUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 3095 | 0.67 | 0.613864 |
Target: 5'- uCUgGCGGCGgcgauauucacggccUGCCGCaGCAUCAUUa -3' miRNA: 3'- -GGgCGUCGUaa-------------ACGGCG-CGUAGUAGg -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 3556 | 0.69 | 0.516098 |
Target: 5'- gUUGCAGCAUUUGUgaaacaCGCuCGUUAUCCc -3' miRNA: 3'- gGGCGUCGUAAACG------GCGcGUAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 20678 | 0.69 | 0.509556 |
Target: 5'- gCCGaAGCAUcUGCCaGUgccuguucugccacgGCAUCAUCCg -3' miRNA: 3'- gGGCgUCGUAaACGG-CG---------------CGUAGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 16924 | 0.69 | 0.494432 |
Target: 5'- aCCUGCucacGCA---GCUGCGCGUCAcgaacuUCCa -3' miRNA: 3'- -GGGCGu---CGUaaaCGGCGCGUAGU------AGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 29464 | 0.69 | 0.494432 |
Target: 5'- uCCUGC-GCAauaucGCCGCGUccguUCGUCCa -3' miRNA: 3'- -GGGCGuCGUaaa--CGGCGCGu---AGUAGG- -5' |
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1073 | 5' | -53.6 | NC_000929.1 | + | 31075 | 1.15 | 0.000355 |
Target: 5'- uCCCGCAGCAUUUGCCGCGCAUCAUCCg -3' miRNA: 3'- -GGGCGUCGUAAACGGCGCGUAGUAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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