miRNA: 3'- cCGaAGCUGCUGGA----GUAGU--UGGUUGU- -5'
miRNA: 3'- -CCGaagCUGCUGG-AGUAGUUGGUUGU- -5'
miRNA: 3'- -CCGa-AGCUGCugGAGU---AGuUGGUUGU- -5'
miRNA: 3'- cCGAAGCUGCUGGAGUaGU--------UGGUUGU- -5'
miRNA: 3'- -CCGAAGCUGCUGGAGUAGUUGGUUGU- -5'
Results 121 - 125 of 125 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10731 | 3' | -49.9 | NC_002794.1 | + | 65902 | 0.75 | 0.843474 |
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Target: 5'- cGCgUCGACGACCUugacCGUCGucGCCGGCGg -3' miRNA: 3'- cCGaAGCUGCUGGA----GUAGU--UGGUUGU- -5' |
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| 10731 | 3' | -49.9 | NC_002794.1 | + | 175435 | 0.76 | 0.809009 |
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Target: 5'- cGGCgcaGACGAUCgUCGUCGAUCAACGu -3' miRNA: 3'- -CCGaagCUGCUGG-AGUAGUUGGUUGU- -5' |
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| 10731 | 3' | -49.9 | NC_002794.1 | + | 56363 | 0.76 | 0.790741 |
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Target: 5'- aGGCggUCGACGugCUCGggaUCcACCGGCAc -3' miRNA: 3'- -CCGa-AGCUGCugGAGU---AGuUGGUUGU- -5' |
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| 10731 | 3' | -49.9 | NC_002794.1 | + | 56848 | 0.85 | 0.353516 |
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Target: 5'- cGCUUCGACGACCUCAaCAagaugagcACCGGCAa -3' miRNA: 3'- cCGAAGCUGCUGGAGUaGU--------UGGUUGU- -5' |
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| 10731 | 3' | -49.9 | NC_002794.1 | + | 150913 | 1.11 | 0.011154 |
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Target: 5'- gGGCUUCGACGACCUCAUCAACCAACAc -3' miRNA: 3'- -CCGAAGCUGCUGGAGUAGUUGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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