Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 10735 | 5' | -58.4 | NC_002794.1 | + | 117905 | 0.67 | 0.795014 |
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Target: 5'- --cGGCCAGCuggUGGAUCGuCuUGAACGUc -3' miRNA: 3'- cucCCGGUCG---ACCUGGCuG-ACUUGCA- -5' |
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| 10735 | 5' | -58.4 | NC_002794.1 | + | 149700 | 0.66 | 0.860338 |
|
Target: 5'- cGGGGGCCgccuGGCgggUGGGCCGcCUGccucgcuacGGCGUg -3' miRNA: 3'- -CUCCCGG----UCG---ACCUGGCuGAC---------UUGCA- -5' |
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| 10735 | 5' | -58.4 | NC_002794.1 | + | 42976 | 0.66 | 0.83713 |
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Target: 5'- --aGGCCAGCUGGugCGcCaGGAUGc -3' miRNA: 3'- cucCCGGUCGACCugGCuGaCUUGCa -5' |
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| 10735 | 5' | -58.4 | NC_002794.1 | + | 114364 | 0.66 | 0.829032 |
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Target: 5'- cAGGGCCGGCUgcaGGGCCGcguaggcCUuGACGUc -3' miRNA: 3'- cUCCCGGUCGA---CCUGGCu------GAcUUGCA- -5' |
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| 10735 | 5' | -58.4 | NC_002794.1 | + | 111498 | 0.66 | 0.83713 |
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Target: 5'- cGAGGGCCAGgacgaGaGACCGGagGGACGUg -3' miRNA: 3'- -CUCCCGGUCga---C-CUGGCUgaCUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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