Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10735 | 5' | -58.4 | NC_002794.1 | + | 149700 | 0.66 | 0.860338 |
Target: 5'- cGGGGGCCgccuGGCgggUGGGCCGcCUGccucgcuacGGCGUg -3' miRNA: 3'- -CUCCCGG----UCG---ACCUGGCuGAC---------UUGCA- -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 157764 | 0.72 | 0.50483 |
Target: 5'- -cGGGCC-GCUGuuucGCCGACUGGugGUg -3' miRNA: 3'- cuCCCGGuCGACc---UGGCUGACUugCA- -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 180390 | 0.68 | 0.768009 |
Target: 5'- --cGGCCAGCUgcucgaacgGGACCGACgucGCGUu -3' miRNA: 3'- cucCCGGUCGA---------CCUGGCUGacuUGCA- -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 189637 | 0.67 | 0.786141 |
Target: 5'- --uGGCUAGCUGGcuggcugacuaGCCGGCUGGccgaGCGg -3' miRNA: 3'- cucCCGGUCGACC-----------UGGCUGACU----UGCa -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 191883 | 0.71 | 0.59916 |
Target: 5'- aGGGGGCggcggaggcgggauCGGCaccgGGACCGGCUGAAuCGg -3' miRNA: 3'- -CUCCCG--------------GUCGa---CCUGGCUGACUU-GCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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