Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
10735 | 5' | -58.4 | NC_002794.1 | + | 56079 | 0.68 | 0.768009 |
Target: 5'- uGAGGGCCAGCaGG-CCGcGCaGcACGUg -3' miRNA: 3'- -CUCCCGGUCGaCCuGGC-UGaCuUGCA- -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 44300 | 0.68 | 0.749418 |
Target: 5'- -cGGGCCGGCgaggcggGGAgCGACcgcGAGCGc -3' miRNA: 3'- cuCCCGGUCGa------CCUgGCUGa--CUUGCa -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 42976 | 0.66 | 0.83713 |
Target: 5'- --aGGCCAGCUGGugCGcCaGGAUGc -3' miRNA: 3'- cucCCGGUCGACCugGCuGaCUUGCa -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 22626 | 0.67 | 0.812333 |
Target: 5'- -cGGGCCGcucgaggcGCUcGGACCG-CUGAuCGUg -3' miRNA: 3'- cuCCCGGU--------CGA-CCUGGCuGACUuGCA- -5' |
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10735 | 5' | -58.4 | NC_002794.1 | + | 15173 | 0.72 | 0.54312 |
Target: 5'- gGAGGGCCGuucGCcgGaGACCGGgUGAACGa -3' miRNA: 3'- -CUCCCGGU---CGa-C-CUGGCUgACUUGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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