Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 10758 | 5' | -62.7 | NC_002794.1 | + | 116638 | 0.67 | 0.601684 |
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Target: 5'- gCGGCagggGCCCGgCCGUCCGgcUCUUg -3' miRNA: 3'- aGUCGgg--CGGGCgGGCGGGCaaAGAA- -5' |
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| 10758 | 5' | -62.7 | NC_002794.1 | + | 78053 | 0.67 | 0.582348 |
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Target: 5'- gUCAGaaCGCCCGCcgCCGCCCGa----- -3' miRNA: 3'- -AGUCggGCGGGCG--GGCGGGCaaagaa -5' |
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| 10758 | 5' | -62.7 | NC_002794.1 | + | 109510 | 0.67 | 0.582348 |
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Target: 5'- gUUGGUCCGCCCgGCCCGCgCuCG-UUCg- -3' miRNA: 3'- -AGUCGGGCGGG-CGGGCG-G-GCaAAGaa -5' |
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| 10758 | 5' | -62.7 | NC_002794.1 | + | 42260 | 0.67 | 0.572726 |
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Target: 5'- -gAGCCCGCuCCGCCCucGCCCc------ -3' miRNA: 3'- agUCGGGCG-GGCGGG--CGGGcaaagaa -5' |
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| 10758 | 5' | -62.7 | NC_002794.1 | + | 131538 | 0.67 | 0.563142 |
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Target: 5'- -gGGCCCGUCaucacgGCCuCGCCCGUggUCg- -3' miRNA: 3'- agUCGGGCGGg-----CGG-GCGGGCAa-AGaa -5' |
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| 10758 | 5' | -62.7 | NC_002794.1 | + | 79781 | 0.68 | 0.52529 |
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Target: 5'- gCGGUUCGCCCGCUaCGCCCGc----- -3' miRNA: 3'- aGUCGGGCGGGCGG-GCGGGCaaagaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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