Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 10760 | 5' | -57.3 | NC_002794.1 | + | 90777 | 0.66 | 0.852695 |
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Target: 5'- cGCUGCUGGUGGaCCUgacgGUCgACcGCGAu -3' miRNA: 3'- uUGACGGCCACC-GGA----CAG-UGaCGUUu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 90331 | 0.66 | 0.844693 |
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Target: 5'- cGCaGCUGGUGGCCgucgGcCugUGCGu- -3' miRNA: 3'- uUGaCGGCCACCGGa---CaGugACGUuu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 114439 | 0.68 | 0.735725 |
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Target: 5'- cAGCUGCCGcacgucGUGGUgCUGUCGCcGCAGc -3' miRNA: 3'- -UUGACGGC------CACCG-GACAGUGaCGUUu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 107093 | 0.68 | 0.715856 |
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Target: 5'- cGACggGCgGG-GGUgUGUCGCUGCGAGc -3' miRNA: 3'- -UUGa-CGgCCaCCGgACAGUGACGUUU- -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 154408 | 0.69 | 0.705808 |
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Target: 5'- --gUGCCGGUGGCCaG-CGCUcGCAu- -3' miRNA: 3'- uugACGGCCACCGGaCaGUGA-CGUuu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 121804 | 0.7 | 0.644512 |
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Target: 5'- cGACcgGCCGGc-GCCUGcCGCUGCAGGa -3' miRNA: 3'- -UUGa-CGGCCacCGGACaGUGACGUUU- -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 28676 | 0.7 | 0.634211 |
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Target: 5'- cGCcGCCGGUGGCC-GUCGCcGCc-- -3' miRNA: 3'- uUGaCGGCCACCGGaCAGUGaCGuuu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 147271 | 0.7 | 0.60333 |
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Target: 5'- gAGCUGCC-GUGGCC-GUCcgGCUGCAc- -3' miRNA: 3'- -UUGACGGcCACCGGaCAG--UGACGUuu -5' |
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| 10760 | 5' | -57.3 | NC_002794.1 | + | 108554 | 1.04 | 0.004263 |
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Target: 5'- gAACUGCCGGUGGCCUGUCACUGCAAAa -3' miRNA: 3'- -UUGACGGCCACCGGACAGUGACGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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