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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
10762 | 3' | -51.6 | NC_002794.1 | + | 91852 | 0.67 | 0.9848 |
Target: 5'- gCACCGUCAaguucgaggguCCcGGGGGUGgcGgcGGCg -3' miRNA: 3'- -GUGGUAGU-----------GGaCCUCCACaaCaaCUG- -5' |
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10762 | 3' | -51.6 | NC_002794.1 | + | 136915 | 0.67 | 0.986528 |
Target: 5'- aCACCGUCAUCgGGAGccccgaGUUGACg -3' miRNA: 3'- -GUGGUAGUGGaCCUCcacaa-CAACUG- -5' |
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10762 | 3' | -51.6 | NC_002794.1 | + | 126558 | 0.68 | 0.976165 |
Target: 5'- aCACCGUCGCCgcGGcGGUGcUGcUGAUc -3' miRNA: 3'- -GUGGUAGUGGa-CCuCCACaACaACUG- -5' |
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10762 | 3' | -51.6 | NC_002794.1 | + | 49123 | 0.68 | 0.967653 |
Target: 5'- gCACCGggACCggccGGAGGUcGUUGUcGACc -3' miRNA: 3'- -GUGGUagUGGa---CCUCCA-CAACAaCUG- -5' |
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10762 | 3' | -51.6 | NC_002794.1 | + | 107483 | 1.11 | 0.00697 |
Target: 5'- cCACCAUCACCUGGAGGUGUUGUUGACg -3' miRNA: 3'- -GUGGUAGUGGACCUCCACAACAACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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